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[ Source: primer3  ]

Package: primer3 (1.1.4-1 and others)

Tool to design flanking oligo nucleotides for DNA amplification

Primer3 picks primers for Polymerase Chain Reactions (PCRs), considering as criteria oligonucleotide melting temperature, size, GC content and primer-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints. All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.

It has been published in Rozen S and Skaletsky H, "Primer3 on the WWW for general users and for biologist programmers.", Methods Mol Biol. 2000;132:365-86.

The Whitehead Institute for Biomedical Research provides a web-based front end to Primer3.

Tags: Field: Biology, Implemented in: C, User Interface: Command Line, Role: Program, Scope: Utility, Supports Format: Plain Text

Other Packages Related to primer3

  • depends
  • recommends
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  • dep: libc0.1 (>= 2.7-1) [kfreebsd-amd64, kfreebsd-i386]
    GNU C Library: Shared libraries
    also a virtual package provided by libc0.1-udeb
  • dep: libc0.3 (>= 2.7-1) [hurd-i386]
    GNU C Library: Shared libraries
    also a virtual package provided by libc0.3-udeb
  • dep: libc6 (>= 2.5-5) [m68k]
    GNU C Library: Shared libraries
    also a virtual package provided by libc6-udeb
    dep: libc6 (>= 2.7-1) [not alpha, hurd-i386, ia64, kfreebsd-amd64, kfreebsd-i386, m68k]
  • dep: libc6.1 (>= 2.7-1) [alpha, ia64]
    GNU C Library: Shared libraries
    also a virtual package provided by libc6.1-udeb
  • sug: ncbi-epcr
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Download primer3

Download for all available architectures
Architecture Version Package Size Installed Size Files
alpha 1.1.4-1 106.8 kB276 kB [list of files]
amd64 1.1.4-1 98.0 kB244 kB [list of files]
arm 1.1.3-1 97.9 kB240 kB [list of files]
armel 1.1.4-1 105.9 kB256 kB [list of files]
hppa 1.1.4-1 103.1 kB240 kB [list of files]
hurd-i386 1.1.3-1 95.5 kB236 kB [list of files]
i386 1.1.4-1 96.5 kB196 kB [list of files]
ia64 1.1.4-1 137.7 kB420 kB [list of files]
kfreebsd-amd64 (unofficial port) 1.1.4-1 101.7 kB212 kB [list of files]
kfreebsd-i386 (unofficial port) 1.1.4-1 97.1 kB198 kB [list of files]
m68k 1.1.1-1 87.7 kB220 kB [list of files]
mips 1.1.4-1 102.5 kB272 kB [list of files]
mipsel 1.1.4-1 102.6 kB272 kB [list of files]
powerpc 1.1.4-1 102.2 kB252 kB [list of files]
s390 1.1.4-1 97.7 kB236 kB [list of files]
sparc 1.1.4-1 97.8 kB248 kB [list of files]