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[ Source: reapr  ]

Package: reapr (1.0.18+dfsg-6)

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universal tool for genome assembly evaluation

REAPR is a tool that evaluates the accuracy of a genome assembly using mapped paired end reads, without the use of a reference genome for comparison. It can be used in any stage of an assembly pipeline to automatically break incorrect scaffolds and flag other errors in an assembly for manual inspection. It reports mis-assemblies and other warnings, and produces a new broken assembly based on the error calls.

The software requires as input an assembly in FASTA format and paired reads mapped to the assembly in a BAM file. Mapping information such as the fragment coverage and insert size distribution is analysed to locate mis-assemblies. REAPR works best using mapped read pairs from a large insert library (at least 1000bp). Additionally, if a short insert Illumina library is also available, REAPR can combine this with the large insert library in order to score each base of the assembly.

Tags: Biology: Nucleic Acids, Field: Biology, field::biology:bioinformatics, implemented-in::c++, User Interface: Command Line, Role: role::program, works-with::biological-sequence

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Download for all available architectures
Architecture Package Size Installed Size Files
amd64 202.3 kB1,069.0 kB [list of files]
arm64 176.0 kB1,221.0 kB [list of files]
armel 168.9 kB847.0 kB [list of files]
armhf 170.8 kB643.0 kB [list of files]
i386 208.9 kB1,035.0 kB [list of files]
mips64el 187.0 kB1,642.0 kB [list of files]
ppc64el 195.4 kB1,669.0 kB [list of files]
s390x 189.9 kB1,001.0 kB [list of files]