Package: primer3 (1.1.4-1)

Tool to design flanking oligo nucleotides for DNA amplification

Primer3 picks primers for Polymerase Chain Reactions (PCRs), considering as criteria oligonucleotide melting temperature, size, GC content and primer-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints. All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.

It has been published in Rozen S and Skaletsky H, "Primer3 on the WWW for general users and for biologist programmers.", Methods Mol Biol. 2000;132:365-86.

The Whitehead Institute for Biomedical Research provides a web-based front end to Primer3.

Tags: Field: Biology, Bioinformatics, Implemented in: C, User Interface: Command Line, Role: Program, Scope: Utility, Supports Format: Plain Text