Package: melting (4.2h-3)

computing the melting temperature of nucleic acid duplex

This program computes, for a nucleic acid duplex, the enthalpy, the entropy and the melting temperature of the helix-coil transitions. Three types of hybridisation are possible: DNA/DNA, DNA/RNA, and RNA/RNA. The program first computes the hybridisation enthalpy and entropy from the elementary parameters of each Crick's pair by the nearest-neighbor method. Then the melting temperature is computed. The set of thermodynamic parameters can be easily changed, for instance following an experimental breakthrough. Melting was published in Le Novère N. (2001), Bioinformatics, 17: 1226-1227.

Tags: Biology: Nucleic Acids, Field: Molecular Biology, Implemented in: C, User Interface: Command Line, Role: Program, Scope: Utility, Purpose: Analysing, Supports Format: Plain Text